Composite

Part:BBa_K3766110:Design

Designed by: Kamyar Keshavarz-Farajkhah   Group: iGEM21_Evry_Paris-Saclay   (2021-09-25)


pTet ABE8.20-m T7RNAP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 777
    Illegal XbaI site found at 706
    Illegal PstI site found at 1266
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 777
    Illegal PstI site found at 1266
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 777
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 777
    Illegal XbaI site found at 706
    Illegal PstI site found at 1266
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 777
    Illegal XbaI site found at 706
    Illegal PstI site found at 1266
    Illegal AgeI site found at 747
    Illegal AgeI site found at 1095
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

to do


Source

DNA synthesis, PCR and assembly

References